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Magnification | Total Magnification | |
---|---|---|

Scanning | 4x | 40x |

Low Power | 10x |
100x |

High Power | 40x | 400x |

Oil Immersion | 100x | 1000x |

To calculate the total magnification of the compound light microscope **multiply the magnification power of the ocular lens by the power of the objective lens**. For instance, a 10x ocular and a 40x objective would have a 400x total magnification. The highest total magnification for a compound light microscope is 1000x.

The total magnification of the image is **the power of the objective lens multiplied by the power of the eyepiece**. For example a 10 eyepiece lens with a 6 • For example, a 10 eyepiece lens with a 6 objective lens produces an overall magnification of 60 (10 x 6).

For example, when using the 40X objective and a 10X ocular, the total magnification would be: **4010=400**.

40x

Scanning Objective Lens (4x) A scanning objective lens provides the lowest magnification power of all objective lenses. 4x is a common magnification for scanning objectives and, when combined with the magnification power of a 10x eyepiece lens, a 4x scanning objective lens gives a total magnification of 40x.Mar 16, 2020

When two lenses are used in combination, the first one forms an image that then serves as the object for the second lens. The magnification of the combination is **the ratio of the height of the final image to the height of the object**.

Grades 1-8 typically will buy a monocular compound microscope with 3 objective lenses: 4x, 10x, 40x for maximum total magnification of **400x**. More advanced students will buy four objectives including a 100x oil immersion objective,. This requires more sophisticated handling with immersion oil.

When you change from low power to high power on a microscope, the high-power objective lens moves directly over the specimen, and the **low-power objective lens rotates away from the specimen**.

All Answers (3) its objective * eyepiece times magnified. So if your objective lens is 5X and eyepiece is 10X then your image is **50 times bigger**. Dear Chandrakant Nirala,The total magnification of a microscope is equal to: (objective lens magnification) * (ocular lens magnification).

**Field of View = Field Number (FN) ÷ Objective Magnification**

You will have to multiply the eyepiece magnification by the objective magnification to find the total magnification before dividing the field number. For instance, if your eyepiece reads 10X/22, and the magnification of your objective lens is 40.

Used for initially bringing specimen into focus. 10x lens magnification, **100x total magnification**.

10X ocular x 40X objective = **400X total magnification**. The distance between the specimen on the stage and the objective lens (how much room you have to move the stage up and down = how much room you have to focus on the image of the specimen).

Total magnification is calculated by multiplying the ocular lens magnification by the objective lens magnification. What is the total magnification of a specimen using the 40X objective? total magnification is **400X**.

Multiply the magnification of the eyepiece by the magnification of the objective lens to produce total magnification. For example, a 10X ocular lens and a 40X objective lens will produce a total magnification of **400X** (10 x 40 = 400).

High Power Objective Lens (40x) The total magnification of a high-power objective lens combined with a 10x eyepiece is equal **to 400x magnification**, giving you a very detailed picture of the specimen in your slide.

**Microscope Magnification**

- At 40x magnification you will be able to see 5mm.
- At 100x magnification you will be able to see 2mm.
- At 400x magnification you will be able to see 0.45mm, or 450 microns.
- At 1000x magnification you will be able to see 0.180mm, or 180 microns.

The answer is **60x times**.

Combination of lenses is used in various optical instruments because: (i) **To increase the magnification of the image.** (ii) To increase the sharpness of the image. (iii) To make the final image erect.

15x x 20x=**300x**. Observing a specimen- more or less light?

There are 3 objective lenses, and each has a different magnification power (10x, **40x**, 100x)The medium power objective lens is 40x.

Objective Lenses: Usually you will find 3 or 4 objective lenses on a microscope. They almost always consist of 4x, **10x, 40x and 100x** powers. When coupled with a 10x (most common) eyepiece lens, total magnification is 40x (4x times 10x), 100x , 400x and 1000x.

In order to find total magnification, the ocular lens magnification, which is **always 10X** for general light compound/optical microscopes, is multiplied with the objective lens magnification, which could either be 4X, 10X, 40X, or 100X.

When switching to the 100x lens, what should you use? 100x lenses should be used with **a few drops of immersion oil** to enhance the image.

**Put the objective lens and nose piece on the lowest power objective**. Turn the coarse adjustment until the nose piece is on the lowest power objective. This keeps the microscope working properly for future use. The lens may look blurry and out of focus.

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