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| Question | Answer |
|---|---|
| associated with a host | grow at body temp, can be pathogenic |
| reservoir | any area that a microbe resides..desk/mouth |
| what was the purpose of incubating the unopened plates. What is an appropriate name for these plates? | sterile media/baseline comparison. Control |
How does growth on the Uninoculated plate affect your interpretation of the other plates?
How does growth on the uninoculated plates affect your interpretation of the other plates? Growth of the uninoculated plates means that there was either an error, contamination , or their before and makes the other plates suspicious. Both plates then probably need to be redone.
How does the growth on the Unopen plate affect the reliability of the other plate?
How does growth on the unopened plates affect the reliability (your interpretation) of the other plates? Contamination when agar was plated could cause contamination in all or just one plate. Growth on unopened plates means the other plates could also be contaminated and the results are not sound.
What was the purpose for incubating the unopened plates plate 7 )?
The purpose of incubating unopened plates is to check in there is contamination in the media preparation phase.
Why were unopened agar plates included in the experiment?
What is an appropriate name for these plates? The unopened plates show sterility of the medium prior to exposure. An appropriate label would be “control.”
What was the purpose of placing an unopened and untreated plate in the incubator?
Terms in this set (46)
What is the purpose of incubating the unopened plates? To use as a contol group. They will not change so one would have enough to control to study off of.
What was the purpose of incubating the Uninoculated plates quizlet?
(Lab 2-1) What is the purpose of incubating the uninoculated plates? The purpose was to have a control group in our experiment. By having untouched plates, we could have examples to compare to our inoculated plates.
What is the likely source reservoir of organisms that grow on plates best at 37 C and how do they survive at room temperature without nutrients?
What is the likely source (reservoir) of organisms that grew at 37℃, and how do you think they survive at room temperature without nutrients? Capture ubiquity of microbes, some grow fast at room them others grow fast at body temp. Source for microbes at 37C= human body.
Why were you asked to incubate the plates at two different temperatures?
The primary reason for incubating bacterial cultures at different temperatures is that specific bacteria are adapted to grow best at different temperatures.
What is the connective tissue and extracellular matrix composed of quizlet?
Connective tissue cells are farther apart than epithelial cells, and they have abundant extracellular matrix between them. This extracellular matrix is composed of protein fibers, and a ground substance consisting of nonfibrous protein and other molecules, and fluid.
What is the point in the lumen White called?
What are the macroscopic structures, which point into the lumen (white), called? intestinal epithelium that extends into the villi. What is the connective tissue and extracellular matrix composed of? of?
What are the microscopic structures which point into the lumen White called?
Villi: the mucosa forms multitudes of projections which protrude into the lumen and are covered with epithelial cells.
What is the purpose of place one Uninoculated plate in each environment tested?
One uninoculated plate from each new lot should also be tested in order to check for contamination of mold or other organisms in the laboratory and/or incubator.
What is the most likely explanation for no growth on a plate or in a tube when growth is expected?
Color of colonies, number of colonies, distribution of colonies on the plate. Results: What would you expect your experimental results to indicate about the effect of ampicillin on the E. coli cells? On which of the plates would you expect to find bacteria most like the original untransformed E.
Which cone of the flame will you use to sterilize the inoculating loop?
Sterilization of inoculating instruments is done in the hottest part of the flame—the tip of the inner cone (red arrow).
Do all bacteria that grow on blood agar break down the blood?
Do all bacteria that grow on blood agar break down the blood? … No, the only thing that grows on blood agar is the bacteria because it breaks down RBCs. Blood agar is a differential medium because all the bacteria will grow on it due to the nutrients and iron.
Where do we dispose of prepared and stained slides of pathogenic bacteria?
Disadvantages: This method is far too risky to use with highly pathogenic organisms. Disposal: Slide and coverslip should be placed in the broken glass biohazard box on the biohazard table.
How can colonies be used to identify bacteria?
Colony morphology is a method that scientists use to describe the characteristics of an individual colony of bacteria growing on agar in a Petri dish. It can be used to help to identify them. A swab from a bin spread directly onto nutrient agar. Colonies differ in their shape, size, colour and texture.
What are control plates?
The control plate does not contain the pGLO plasmid, but the other variables are the same. The control plate proves that the transformation is dependent on the presence of the pGLO plasmid. … All 3 transformation plates contain genetically transformed bacterial cells because they have taken up the pGLO plasmid.
Why do we put the plates in the incubators at 25 C and not higher?
Inoculated agar plates are incubated at 25°C in school laboratories for no more than 24–48 hours. This encourages growth of the culture without growing human pathogens which thrive at body temperature (37°C).
What is a petri dish used for in science?
Petri dishes are commonly used to study microorganisms in laboratories. It’s a shallow round glass that’s cylindrical in shape, particularly important to growing cultures of cells, viruses, bacteria, and other microorganisms.
What is Uninoculated tube?
In a microbiology lab, an uninoculated tube is a negative control, from which no reactions are expected.
Why is it important to carefully insert and remove the needle along the same stab line?
Why is it important to carefully insert & remove the needle along the same stab line? Because a lateral movemnt of the needle will make interpretation more difficult.
What is the likely source reservoir for the organisms that grew best at 25ºc What is the likely source reservoir for the organisms that grew best at 37ºc?
The source for organisms at 25ºC is likely outside, such as in soil. For 37ºC, the likely source is the human body.
Why might the amount or type of growth seen on the plates of two different groups that sampled the same site be different?
Why might the amount or type of growth seen one the plates of two different groups that sampled the same sight be different? The might contain diff. types of bacteria that might not grow a lot due to the nutrients and the temperature it was stored in. Also diff.
Which agent seemed to be least effective at removing microbes from the fingertips?
Which agent seemed to be least effective at removing microbes from the fingertips? Hibiclens was the least effective at removing microbes because about 3 different types of bacteria grew on my plate.
Why do we grow E coli at 37 C?
E. coli is a mesophile that grows best at 37 degrees Celsius in neutral pH environments. E. coli is a facultative aerobe and is able to grow without oxygen, but it can extract more energy from its nutrient source and grow faster if oxygen is present.
What do we use to inoculate our agar plates and liquid broth?
Inoculating loops and needles made of metal wire can be used to transfer bacteria from one medium to another, such as from the surface of an agar plate to a broth. Metal tools may be sterilized by heating them in the flame of a Bunsen burner.
What was the purpose of using a filter paper disc soaked in distilled water?
The paper disc soaked in distilled water is a control. It shows us that any change in the starch is due to what we have soaked into the papers, not to the paper discs themselves or any other aspect of the technique.
The extracellular matrix, called plasma, makes blood unique among connective tissues because it is fluid.
Which tissue type arises from all three?
epithelial tissue
Note that epithelial tissue originates in all three layers, whereas nervous tissue derives primarily from the ectoderm and muscle tissue from mesoderm.
What are connective tissues?
Tissue that supports, protects, and gives structure to other tissues and organs in the body. … Connective tissue is made up of cells, fibers, and a gel-like substance. Types of connective tissue include bone, cartilage, fat, blood, and lymphatic tissue.
What is bowel lumen?
Intestinal Lumen
In the intestines, the lumen is the opening inside the bowels. It is surrounded by the other parts of the intestinal wall: the mucosa, the submucosa, the muscularis, and the serosa.
What are villi?
